7. In Figure 4, the authors induced apoptosis, a form of regulated cell death, in MCF-7 cells by treatment with H2O2 and PbIV
A. In parts of Figure 4, the authors utilized an shRNA technique to reduce the expression of neuroglobin. Describe how the data shown in Figure 4B acts a ‘control’ for this technical approach. (2 marks)
B. Increases in apoptosis is observed by the presence of the PARP-1 cleavage product (seen as the 86kDa protein band on immunoblots). Use the data in Figures 4C and 4D to explain whether apoptosis triggered by H2O2 and PbIV is affected by changes in neuroglobin expression. (Use the general approach for describing a data figure that was discussed in class). (2 marks)
C. What is the main difference in the experimental design used in Figures 4E and 4F compared to 4C and 4D? Are the results in 4E/4F different from those seen in 4C/4D? Briefly explain. (2 marks)
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4858147/ <<---(FULL ARTICLE)
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